evaluation
-
Run into the paper statistics, helpless. On Qimo, I saw that Dr.Tong company provided the assistance of thesis statistics, and with the guidance and assistance of the doctor's teacher, I ran smoothly to get the results. Thank Dr.Tong for his high-level service.
Mr. Lin
from: tainan
-
When a doctoral dissertation encounters multiple linear regression, it has always been a difficult point and there is nothing to do. On Google, we can see that the doctor teacher of Dr.Tong statistics company provides statistical running and statistical guidance. The information and requirements to the doctor teacher, with the help of the teacher, smoothly run out the results. Thank you for the help and guidance from the doctor of Dr.Tong statistics company.
Dr. Li
from: taoyuan
-
When I met the difficulties in the statistical analysis of the doctoral thesis questionnaire, I couldn't find a clue. Finally, I saw Dr.Tong's assistance in the statistical analysis of the thesis questionnaire on qimo.com. With the assistance of the doctor, I got the results smoothly. Thank Dr.Tong for his assistance in the statistical analysis of the thesis questionnaire, which solved my urgent problem.
Dr. Li
from: Kaohsiung
-
Previously, a local statistics company in Hong Kong was entrusted, but it failed to do a good job in medical statistics. In my anxiety, I saw the doctor teacher of Dr.Tong statistics company provide statistical running and guidance, and sent the data and requirements to the doctor teacher. With the efforts of the doctor teacher, the analysis results were successfully made. I feel that Taiwan's statistics companies are more professional and the fees are reasonable.
Dr. Li
from: hongkong
-
Thank you for the efficient and high quality service provided by the doctor teacher of Dr.Tong statistics company. Solved my urgent need.
Mr. Lin
from: yunlin
-
When I met the difficulties in the statistical analysis of the pre-test and post-test of the thesis questionnaire, I couldn't solve them all the time. On Qimo, I saw that the doctoral teacher of Dr.Tong statistics company provided the tutoring service of thesis statistics, and sent the information and requirements to the doctoral teacher. With the help and guidance of the teacher, I got the results. Thank you very much for the tutoring service of the doctoral teacher of Dr.Tong statistics company There are two difficulties.
Miss Liu
from: Kaohsiung
-
It is difficult to analyze English papers by SPSS. On Google, we can see that the doctoral teacher of Dr.Tong statistics company provides the paper statistics running and guidance, sends the data and analysis requirements to the teacher, communicates with the teacher, and runs smoothly with the teacher's guidance and help. Thank you very much for the help of the doctor teacher of Dr.Tong statistics company. The teacher is serious, responsible and powerful.
Miss Zeng
from: london
-
It's difficult to analyze Stata data in accounting papers. On Yahoo Qimo, I saw the doctor teacher of Dr.Tong statistics company provide Stata statistical running guidance. The data and requirements were sent to the doctor's teacher. Under the guidance of the doctor's teacher, the Stata code was successfully designed, and the analysis results were also run out. I would like to thank the doctor's teacher of Dr.Tong statistics for his high level guidance and assistance.
Mr. Zhang
from: taizhong
-
Educational papers have quantitative statistics, which is a bit difficult! Helpless, I saw on Yahoo Qimo that Dr. and postdoctoral teachers from Dr.Tong Statistics Company provide doctoral thesis statistics and counseling services. I sent the questionnaire and sample data to the doctor, and with the guidance and assistance of the doctor, I successfully obtained the results and graduated. I am very grateful for the professional guidance of the doctor and postdoctoral teacher from Dr.Tong Statistics Company. The technical expertise, reliable quality, and reasonable fees have saved me a lot of trouble.
Dr. Song
from: Kaohsiung
|
|
|
|
| 2023-10-23 14:08:32 | onclick： | Scientists create first engineered exosomes for aquatic animals |
 |
|
Recently, Shao Changwei, a researcher at the National Key Laboratory of Aquaculture Biological Breeding and Sustainable Production at the Yellow Sea Institute of Fisheries Research, China Academy of Fisheries Sciences.This is the first development and application of engineered exosomes in aquatic animals, which will play an important role in the precise regulation of gene function and reproductive stem cell transplantation.The research results were published in the journal Controlled Release in the field of drug delivery.
Exosomes are extracellular vesicles that can be secreted by most cells. The diameter is about 100 nanometers. Because of their advantages of low immunogenicity and low toxicity, exosomes have become very promising functional molecules and drug delivery carriers.Shao Changwei's team successfully constructed an engineered exosome by bioinformatics, molecular and cell biological experiments, using the conserved sex-critical gene dMRt1 as the regulatory object.
The engineered exosomes construction technology system mainly includes three parts: screening of intranets, determination of target peptides and preparation of engineered exosomes.
The inclusion screening refers to the screening of miRNAs that can effectively inhibit the expression of dMRT1 sex-determining genes through experimentally.In order to determine the target peptide, we first select the receptor with high expression in the spermatid cell membrane by means of single cell sequencing and full transcriptome sequencing.The exosomes of ovary cells were extracted by differential hypercentrifugation, and the candidate peptides were loaded onto the surface of exosomes by phospholipid membrane anchoring technique.Finally, the expression of dMRT1 in spermatogenesis was inhibited by using the engineered exosome in vivo, which proved that dMRT1 plays an important role in maintaining spermatogenesis.
The establishment of the technology system not only controls the function of sex-related genes, but also applies to the target transplantation of fish germ stem cells.
The study is another important landmark in the field of epigenetics of fish sex determination and differentiation following the team's first confirmation of the regulatory mechanism of cRNA and the cytological basis of fish sex generation.
The research was supported by the National Key R&D Plan, the National Natural Science Foundation Youth Science Foundation, the Taishan Scholars' Young Experts, the National High-level Talents' Special Support Plan.
|
|
 |
|
|
|
|
|
